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During the course of the reaction, monitoring on TLC, you will see that the hat (nitrostyrene product) will become brighter and the head (3,4,5- TMBA) will fade. 16) From the freezer collect the second crop of crystals. Some more have fallen out, but it is a different colour, bit more greenish-yellow and darker, but they might be more dense. For some reason wash with 10% sodium bisulfite sol. to see what happens, no adduct is formed.